Alternative buyer's shipping account are also welcome CANADA: A Minimum Shipping Charge of $20.00usd will be applied to all auctions. IMPORTANT: Read our shipping instructions allocated to your region Canada. Groupe Interconnexion reserves the right to open an unpaid auction request.Unless otherwise notified) All Items are subject to a Packing and Handling Fee of 3% 15$ Minimum) Canadian residents should expect Provincial/Federal taxes to be applied. In the case that payment has not been received within 7 working days. Payment must be received no later than 7 working days from the closing date of the auction. Item ID: 405915, Manufacturer: Gilson, Model Number: Microman, Description: Lot of 6 Pipettes, Weight: 1Lbs, Condition: Tested, Cosmetic: Very Good, Have questions regarding this product? Contact us: Bidders with less than a( 5) rating must contact us prior to bidding otherwise their bids will be removed. Includes 2 x M25 Pipettes(3-25 uL) 2 x M50 Pipettes(20-50 uL) 2 x M250 Pipettes(50-250 uL) NO RESERVE! BUY IT NOW Ask our Sales Personnel for details. Positive-displacement pipette that, together with its Capillary Pistons(CPs) will ensure accuracy and precision when pipetting problem liquids, and offer full protection against cross contamination. The correction factor is 0.2% per degree of difference between the sample temperature (T sample) and 20 ☌.Gilson_microman_lot_of_6_pipettes Gilson’s MICROMAN is a fully adjustable. This produces results comparable to those of the second pipetting cycle.Īlternatively, we determined a theoretical correction factor that can be used to adjust the volume of Gilson Pipetman pipettes according to the sample temperature. The technique reduces the temperature difference between the sample and the air cushion, and it enhances precision without affecting accuracy. One technique, prewetting, requires aspirating and dispensing the sample liquid three or four times before aspirating for sample delivery. If this is not possible, researchers can use certain techniques to offset the impact of temperature. We recommend that liquids and pipettes be allowed to equilibrate to ambient temperature. The more the temperature gap widens, the greater the gravimetric errors become. Our findings indicate that accuracy (systematic error) drifts by as much as 0.1–0.2% per degree of difference between the sample temperature and 20 ☌. Reproducible results (limitation of random error) within the thresholds defined by the manufacturer's precision specifications cannot be obtained ( Fig. ![]() 2), and that for hot samples stabilizes above the standard value, but the volume continues to fluctuate, albeit at a considerably lower rate. During the second aspiration, the air cushion travels from the warm area of the tip that was previously in contact with the water, to the tepid portion of the tip holder, which then causes a slight drop in the temperature and results in the air volume shrinking, producing a higher-than-expected delivery volume.įrom the third cycle onward, the delivered volume for cold samples stabilizes below the standard value ( Fig. As a result, the sample volume dispensed is lower than what is expected under standard conditions. The first time water at 40 ☌ is aspirated, the temperature of the air cushion that is in contact with the water rises causing the air to fill a greater volume. We also observed a sharp inversion of this phenomenon in both cases as of the second pipetting cycle ( Fig. Conversely, when we dispensed a 40 ☌ sample for the first time, the volume delivered was less than the reference. When we aspirated a 4 ☌ sample for the first time, we observed that the dispensed volume was always greater than that obtained under standard conditions. We performed ten measurements each, without changing tips, of water at the two temperatures, using the Pipetman P100 with Gilson Diamond® tips and volume set at 100 μl in a 20 ☌ regulated environment. ![]() We compared sample volumes in consecutive pipetting cycles of distilled water at 4 ☌ and 40 ☌ with volumes expected at 20 ☌, the standard reference temperature for calibrating pipettes. The work was performed in our ISO 17025–accredited laboratory. Here we report the results and offer solutions to circumvent the issue. Gilson conducted a study to determine the influence of sample temperature on the performance of Microman and Pipetman pipettes. For example, pipetting hot samples, while they are cooling after their incubation at 37 ☌, has the opposite effect. Oddly, a smaller volume is delivered from the second aspiration onward if the tip is not changed. Observant scientists will have noticed that when they pipette cold samples, they invariably aspirate a larger-than-expected sample volume each time the tip is changed.
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